Xin Chen Lab
Research laboratory of Xin Chen, PhD
Liver Cancer Laboratory
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| Sample | RNA amount add (1ug) | DEPC water (ul) | Primer (Oligo(dT)) (ul) | 10mM dNTP mix (ul) | Total |
| A | x ul | 9-x ul | 1 | 2 | 12 |
65°C for 5 min in PCR machine
4°C in PCR machine
Master Mix
| 1X | 20X | |
| 5x cDNA Synthesis buffer (vortex) | 4ul | 80ul |
| 0.1M DTT | 1ul | 20ul |
| RNAse OUT | 1ul | 20ul |
| DEPC-H2O | 1ul | 20ul |
| ThermoScript RT | 1ul | 20ul |
| Total | 8ul | 160ul |
Add 8ul of the Master Mix to each sample
50°C for 30min and 85°C for 5 min in PCR machine.
Store at -20°C
FOR ALL PATIENT-RELATED MATTERS
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Telephone: (808) 586-3010
Email: info@cc.hawaii.edu
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EMPLOYMENT OPPORTUNITIES
Email: hr@cc.hawaii.edu
