Protocols

Cell Biology

Molecular Biology

Chen Lab plasmids available to Addgene
Cloning
Digest map of Plasmids
Common Primer Sequences
Common used primer in Chen lab
CRISPR cloning notes and sgRNA design
CRISPR cloning protocol Zhang Lab
Extract DNA from Paraffin Blocks

Identify sgRNA 2022
PX330 cloning protocol
Quantitative Microsatellite Analysis
RT-PCR
shRNA template for PT3 cloning
TaqMan RT
Thermoscript RT

Bioinformatics Analysis

Useful web tools for TCGA data analysis
Identification of promoter sequence

Biochemistry

Protein Extraction
Western Blotting

Histology

BrdU staining
H&E staining
IF on frozen sections (double IF, for 2 rat antibodies)
IF on frozen sections (double IF)
IF paraffin slides (double staining)

IF paraffin slides
IHC rabbit antibody protocol
IHC mouse antibody protocol
Oil red O staining
PicroSirius red staining

Animal

Alcohol feeding protocol
Alpelisib formulation
Blood analysis for liver panel
Cabozantinib formulation
DEN protocol
DDC protocol
Gemcitabine formulation
Genotyping
Harvest mouse liver tissues
Hydrodynamic tail vein injection

INK128 formulation
Liver tumor organoid protocol
Mice Super-Ovulation Protocol
Protocol for weaning mice
Rapamycin mice
Spleen injection
Sorafenib formulation
Tamoxifen injection
Tunicamycin treatment

Other protocols

Chemical SOP: Hydrochloric Acid
Image modification for making figures